Location:Hyderabad, India (From home)Education level: Masters
Education field: Biological and Biomedical Sciences
Years of experience: more than 5
Attributes that best describes:
I have research experience and is familier with terms in biotech.know Molecular Biology: Genomics and proteomics.know the techniques like
Isolation and handling of genomic DNA from blood, plant tissues, bacteria, phage and forensic sources. Isolation of bacterial Plasmid DNA and their quantification.
Primer designing and polymerase chain reaction (PCR).
Genotyping using STR and RAPD markers.
Automated DNA Sequencing.
Site directed mutagenesis, Random mutagenesis.
Gene cloning, screening of recombinants.
Genomic DNA library construction.
Protein purification, Concentration, Dialysis, Quantification and pull down experiments.
Gel exclusion chromatography and protein purification using AKTA.
Western blot, Antibody purification using poly A and cross linking.
Agarose and SDS-PAGE.
Microbiology: Bacteriology and virology
Bacterial transformation, transduction, electroporation, electroduction and genetic screening.
Chemical, ultra and electrocompetent cell preparation.
Mycobacteriphage culturing, Plaque purification, viral titer enhancement, Cryopreservation.
Glycerol and DMSO stock maintenance of bacteria and phage.
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