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Location:Hyderabad, India (From home)

Education level: Masters
Education field: Biological and Biomedical Sciences
Years of experience: more than 5
Attributes that best describes:
Independent worker
Good communicator

I have research experience and is familier with terms in biotech.know Molecular Biology: Genomics and proteomics.know the techniques like
 Isolation and handling of genomic DNA from blood, plant tissues, bacteria, phage and forensic sources. Isolation of bacterial Plasmid DNA and their quantification.
 Primer designing and polymerase chain reaction (PCR).
 Genotyping using STR and RAPD markers.
 Automated DNA Sequencing.
 Site directed mutagenesis, Random mutagenesis.
 Gene cloning, screening of recombinants.
 Genomic DNA library construction.
 Protein purification, Concentration, Dialysis, Quantification and pull down experiments.
 Gel exclusion chromatography and protein purification using AKTA.
 Western blot, Antibody purification using poly A and cross linking.
 Agarose and SDS-PAGE.

Microbiology: Bacteriology and virology
 Bacterial transformation, transduction, electroporation, electroduction and genetic screening.
 Chemical, ultra and electrocompetent cell preparation.
 Mycobacteriphage culturing, Plaque purification, viral titer enhancement, Cryopreservation.
 Glycerol and DMSO stock maintenance of bacteria and phage.

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